Journal: Science Advances

Article Title: Carbonic anhydrase VIII regulates IP3R1-dependent Ca 2+ signaling to coordinate insulin secretion and hepatic glucose production

doi: 10.1126/sciadv.aeh4948

Figure Lengend Snippet: CA8 negatively regulates GIIS and HGP by inhibiting IP3R1-mediated Ca 2+ release from ER. This characteristic implies that CA8 is involved in orchestration of glucose homeostasis. Altered CA8 expression in islets and hepatocytes of ob/ob mice is accompanied with lower GIIS and elevated HGP, respectively. Furthermore, chronic exposure of glucagon and exendin-4 elevated CA8 expression, consequently limited HGP in vivo. Created in BioRender. Murakami, T. (2026) https://BioRender.com/qpiyzwk .

Article Snippet: s63273, s63274, s63275, Ambion, TX, USA) or IP3R1 (25 μM, catalog no. sc-42476, Santa Cruz Biotechnology, TX, USA) was used.

Techniques: Expressing, In Vivo

Journal: Nucleic Acids Research

Article Title: Mitochondrial DNA replication is regulated by endoplasmic reticulum-mitochondrial contact sites, the mitochondrial calcium uniporter, and manganese

doi: 10.1093/nar/gkag233

Figure Lengend Snippet: ERLIN2 silencing depletes ER-mitochondrial junctions. ( A ) Antibodies to ER-mitochondrial junction components IP3R1 and GRP75 individually label the ER and the mitochondria, respectively, verified by KDEL labelling the ER and TOMM20 the mitochondria. Scale bars = 30 µm. ( B ) RTN4 and ERLIN2 silencing diminished the GRP75/IP3R1 PLA signal, quantified in 121 non-target (NT) cells versus 91 RTN4 and 61 ERLIN2 silenced cells ( n = 3–6 independent experiments, P = .002). Scale bars = 50 µm.

Article Snippet: Cells were then incubated with primary antibodies GRP75 (Santa Cruz Biotechnologies) and IP3R1 (NOVUS) in Antibody Diluent overnight at 4°C.

Techniques: